Coding

Part:BBa_K2684001

Designed by: Yihao Zhang   Group: iGEM18_SHSBNU_China   (2018-10-08)


PelB-CotA

CotA laccase attached with PelB signal peptide sequence

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 1411
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 349


Usage and Biology

PelB is a signal peptide attached to CotA coding sequence. PelB signal peptide can help transfer CotA out of E.coli.

The enzyme activity was measured and calculated based on the weight of our sample. Formula: laccase activity (nmol/min/g) = ΔA /ε(ABTS mmolar extinction coefficient) / d * V (total volume of reaction) / V (volume of sample in the reaction, 0.025mL) / W (sample mass, g) * V (extracted liquid added, 1mL) / T (reaction time, 3 minutes) = 130 *ΔA / W.

For more experimental details, please visit http://2018.igem.org/Team:SHSBNU_China/Experiments

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